Assessment of Status of rpoB Gene in FNAC Samples of Tuberculous Lymphadenitis by Real-Time PCR

Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

Background & Aim: Pseudomonas aeruginosa strains that were resistance to majority of commonly used antibiotics were caused problem in treatment of these infections. Imipenem is the excessive potential antibiotic for elimination of antibiotic resistance isolates of these bacteria. Aim of this study was, identification of imipenem effect on TEM beta-lactamase gene expression in resistant to antib...

Role of Fine Needle Aspiration Cytology in Tuberculous Lymphadenitis

Tuberculous lymphadenitis continues to be one of the most common causes of lymphadenitis. FNAC avoids the physical and psychological trauma occasionally encountered after biopsy, general anaesthesia, a surgical operation and hospitalisation. We wanted to study the utility and limitations of FNAC in tuberculous lymphadenitis. Total 1272 FNAC cases with enlarged superficial lymphnodes were includ...

Study of the role of HOTAIR gene on colorectal cancer using Real-time PCR

BBackground: Despite advances in cancer studies, colorectal cancer, as the third most common cancer, has the highest mortality rate worldwide. Due to its high prevalence in the younger ages and advanced stages, screening of this cancer with molecular methods is necessary. Studies have shown that HOTAIR gene plays an important role in cancers. Our aim in the present study was to determine the...

Mutations in the rpoB Gene of Rifampin Resistant Myco-bacterium tuberculosis Isolated in Isfahan by PCR-SSCP

Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples

During 2010-2011, Real-time PCR procedure was used to detecting FMDV RNA on 147 epithelium samples from the field. In this survey, for Real-time PCR from 3D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our convention...